Mapping the developing human immune system across organs
Single-cell genomics studies have decoded the immune cell composition of several human prenatal organs, but were limited in understanding the developing immune system as a distributed network across tissues. We profiled nine prenatal tissues combining single-cell RNA sequencing, antigen-receptor sequencing and spatial transcriptomics to reconstruct the developing human immune system. We revealed late acquisition of immune effector functions of myeloid and lymphoid cell subsets, and the maturation of monocytes and T cells prior to peripheral tissue seeding. We uncovered system-wide blood and immune cell development beyond primary hematopoietic organs, characterized human prenatal B1 cells, and shed light on the origin of unconventional T cells. Our atlas provides both valuable data resources and biological insights that will facilitate cell engineering, regenerative medicine and disease understanding.
- Contact
- Emma Dann
- DOI
- 10.1126/science.abo0510
- Release
- 12 May 2022
- Lab
- Teichmann Lab
- Tissue
- Fetal Bone Marrow, Fetal Gut, Fetal Kidney, Fetal Liver, Fetal Mesenteric Lymph Node, Fetal Skin, Fetal Spleen, Fetal Thymus, Organoids, Yolk Sac
- Assay
- 10x 3' v2, 10x 5' v1, 10x 5' v2, Visium
- Disease
- None
- Organism
- Homo sapiens
scRNA-seq Datasets
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
10x 3' v2
Spatial Datasets
VDJ Datasets
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
Fetal Gut
Fetal Kidney
Fetal Liver
Fetal Mesenteric Lymph Node
Fetal Skin
Fetal Spleen
Fetal Thymus
Yolk Sac
Reproducibility
Reproducibility is a major principle underpinning the scientific method. We make publicly available the raw data and analysis scripts associated with each collection.
- Code Repository
- https://github.com/Teichlab/Pan_fetal_immune
