A cell atlas of human thymic development defines T cell repertoire formation
The human thymus is the organ responsible for the maturation of many types of T cells, which are immune cells that protect us from infection. However, it is not well known how these cells develop with a full immune complement that contains the necessary variation to protect us from a variety of pathogens. By performing single-cell RNA sequencing on more than 250,000 cells, Park et al. examined the changes that occur in the thymus over the course of a human life. They found that development occurs in a coordinated manner among immune cells and with their developmental microenvironment. These data allowed for the creation of models of how T cells with different specific immune functions develop in humans.
- Contact
- Muzlifah Haniffa
- DOI
- 10.1126/science.aay3224
- Release
- 21 February 2020
- Lab
- Haniffa Lab
- Tissue
- Liver, Thymus
- Assay
- 10x 3' v2, 10x 5' v2
- Disease
- None
- Organism
- Homo sapiens, Mus musculus
scRNA-seq Datasets
Reproducibility
Reproducibility is a major principle underpinning the scientific method. We make publicly available the raw data and analysis scripts associated with each collection.
- Raw Data
- https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-8581/
- Code Repository
- https://zenodo.org/record/5500511