A cell atlas of human thymic development defines T cell repertoire formation

The human thymus is the organ responsible for the maturation of many types of T cells, which are immune cells that protect us from infection. However, it is not well known how these cells develop with a full immune complement that contains the necessary variation to protect us from a variety of pathogens. By performing single-cell RNA sequencing on more than 250,000 cells, Park et al. examined the changes that occur in the thymus over the course of a human life. They found that development occurs in a coordinated manner among immune cells and with their developmental microenvironment. These data allowed for the creation of models of how T cells with different specific immune functions develop in humans.

Contact
Muzlifah Haniffa
DOI
10.1126/science.aay3224
Release
21 February 2020
Lab
Haniffa Lab
Tissue
Liver, Thymus
Assay
10x 3' v2, 10x 5' v2
Disease
None
Organism
Homo sapiens, Mus musculus

scRNA-seq Datasets

Dataset
Tissue
Assay
Disease
Organism
Count
Thymus
10x 3' v2
10x 5' v2
None
Homo sapiens
255901
Liver
Thymus
10x 3' v2
10x 5' v2
None
Homo sapiens
30694
Thymus
10x 3' v2
10x 5' v2
None
Homo sapiens
76994
Thymus
10x 3' v2
10x 5' v2
None
Homo sapiens
18524
Thymus
10x 3' v2
10x 5' v2
None
Mus musculus
6811
Thymus
10x 3' v2
10x 5' v2
None
Mus musculus
36084

Reproducibility

Reproducibility is a major principle underpinning the scientific method. We make publicly available the raw data and analysis scripts associated with each collection.

Human Cell Atlas

Human Cell Atlas

Developmental

The Human Developmental Cell Atlas (HDCA) aims to generate a comprehensive profile of cell types and states present during development. This detailed study of development will be critical for understanding congenital and childhood disorders, as well as ageing.

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